FOR LABORATORY RESEARCH USE ONLY (RUO)
Methylene blue (3,7-bis(dimethylamino)phenothiazin-5-ium chloride trihydrate) is a synthetic thiazine dye provided as a high-purity, USP-grade powder for exclusive use in analytical and experimental procedures within controlled laboratory settings. This compound serves as a versatile reagent for in-vitro applications such as biological staining, spectrophotometric assays, and redox titrations. Product Specifications:
- CAS Number: 61-73-4 (anhydrous); 7220-79-3 (trihydrate)
- Molecular Formula: C16H18ClN3S • 3H2O
- Molecular Weight: 373.90 g/mol (trihydrate)
- Purity: ≥98% (HPLC; USP grade)
- Appearance: Dark green crystalline powder; yields deep blue solution in water (λmax: 664 nm)
- Solubility: Soluble in water (43.6 mg/mL at 20°C), ethanol, and chloroform; insoluble in ether
- pH (1% aq. solution): 3.0 – 4.5
- Storage: Keep tightly closed in a cool, dry place (15-30°C; 59-86°F); protect from light to prevent degradation
- Packaging: Amber glass vial (1g net weight) with desiccant for stability
- Certificate of Analysis (COA): Available upon request—includes lot-specific purity, heavy metals (<10 ppm), and residual solvents testing
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